Where is Cre in a neuron?
Cre is expressed in layer 6 corticothalamic neurons in an ntsr1 BAC-Cre line (B, Ntsr1). Clearly labeled are the projection axons of these neurons, which terminate in the dorsal thalamic nuclei.
What is Cre recombination?
Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus.
What is nestin Cre?
This strain is commonly known as nestin-Cre. Nestin promotor driven Cre recombinase is expressed in the central and peripheral nervous system, including neuronal and glial cell precursors. Hemizygous nestin-cre mice exhibit mild hypopituitarism, growth retardation, impaired fear response, and a metabolic phenotype.
How does Mx1 Cre work?
The Cre recombinase is under the control of the Mx1 promoter, which can be induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA (such as poly I:C); enabling the “knockout” at any time during development in progeny of a floxed mutant mated to …
How contagious is Cre?
How are CRE germs spread? CRE are usually spread person to person through contact with infected or colonized people, particularly contact with wounds or stool (poop). This contact can occur via the hands of healthcare workers, or through medical equipment and devices that have not been correctly cleaned.
What is Cre used for?
Cre recombinase plays important roles in the life cycle of the P1 bacteriophage. Upon infection of a cell the Cre-loxP system is used to cause circularization of the P1 DNA. In addition to this Cre is also used to resolve dimeric lysogenic P1 DNA that forms during the cell division of the phage.
What are Cre mice used for?
The Cre/lox system is one of the most powerful and versatile tools developed for mouse genetics. It gives mouse researchers sophisticated control over the location and timing of gene expression. Cre/lox is usually used to make knockout alleles, but it can also be used to activate gene expression.
Where is Emx1 expressed?
Abstract. The homeobox-containing gene, Emx1, a mouse homologue of Drosophila empty spiracles, is specifically expressed in the developing telencephalic cortex.
What are LSK cells?
KSL cells are an early form of mouse/murine hematopoietic stem cells. HSCs [Hematopoietic stem cells] in murine cultures show phenotypic markers as being CD34-, CD150+, and Flt3- for LTR [long-term reconstitution]. Kit (CD117) is the receptor of Stem Cell Factor. Sca-1 is a murine hematopoietic stem cell antigen.
How is Cre expression directed to motor neurons?
Under control of the endogenous upstream elements, cre expression is directed to motor neurons. In heterozygotes, cre expression coincides with HB9 expression. Homozygous HB9 cre mice die at or soon after birth, with expression of Cre recombinase likewise directed to motor neurons but no expression of endogenous HB9.
Where is Cre expression found in a mouse?
Expression is specific to Cd4+Cd25 Cd127 T cells from the lymph nodes, spleen and thymus. Expression also seen in ovary. GFAP-Cre transgenic mice from founder line 73.12 have Cre recombinase expression directed by the mouse glial fibrillary acidic protein promoter.
How does HB9 knock in mutation affect Cre expression?
Mice heterozygous for this HB9 cre (now Mnx1tm4 (cre)Tmj ) knock-in mutation are viable and fertile, with cre expression replacing HB9 ( Hlxb9 or Mnx1) expression. Under control of the endogenous upstream elements, cre expression is directed to motor neurons. In heterozygotes, cre expression coincides with HB9 expression.
Where does GFAP-CRE transgenic mice have Cre expression?
GFAP-Cre transgenic mice from founder line 73.12 have Cre recombinase expression directed by the mouse glial fibrillary acidic protein promoter. Cre expression is observed in astrocytes in the brain and spinal cord, as well as postnatal and adult GFAP-expressing neural stem cells and their progeny in the brain.