Where does KpnI cut?
Where does KpnI cut?
Description. Thermo Scientific KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
What is Kpnl?
KpnI is a DNA restriction enzyme that is used in molecular biology research to cleave DNA at the recognition sequence 5′-GGTAC/C-3′ to generate DNA fragments with 3′-cohesive termini.
What consensus sequence does HincII cut?
Description. Thermo Scientific HincII (HindII) restriction enzyme recognizes GTY^RAC sites and cuts best at 37°C in Tango buffer.
Is restriction enzyme inactivation necessary?
Inactivation of restriction endonucleases is generally not necessary, but in some cases it might increase the transformation efficiency.
Why do you need to inactivate restriction enzyme before ligation?
Incomplete inactivation of the restriction enzyme can cause ligation to fail. Follow the inactivation protocol per the manufacturer. For some enzymes that cannot be heat inactivated, purification is required.
What is H in Bam h1?
BamHI (from Bacillus amyloliquefaciens) is a type II restriction endonuclease, having the capacity for recognizing short sequences (6 bp) of DNA and specifically cleaving them at a target site. This allows the DNA to maintain its normal B-DNA conformation without distorting to facilitate enzyme binding.
How much does one unit of DPNI take?
Bulk packaging may also be available and requested for large recurring orders. An E. coli strain that carries the DpnI gene from Diplococcus pneumoniae G41 (S. Lacks). One unit is defined as the amount of enzyme required to digest 1 µg of pBR322 DNA ( dam methylated) in 1 hour at 37°C in a total reaction volume of 50 µl.
Which is the highest purity KPNI or Neb?
Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. KpnI has a High Fidelity version KpnI-HF ® ( NEB #R3142 ).
Which is an example of a DPNI unit?
Properties & Usage Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of pBR322 DNA (dam methylated) in 1 hour at 37°C in a total reaction volume of 50 µl. Product Notes DpnI cleaves only when its recognition site is methylated.
How big is an isoschizomer of KpnI?
One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Acc65I is an isoschizomer of KpnI.