What is rhodamine 123 used for?
What is rhodamine 123 used for?
Rhodamine 123, a member of the rhodamine family of flurone dyes, has been used to examine membrane transport by the ABCB1 gene product, MDR1. MDR1 is viewed as the archetypal drug transport protein, and is able to efflux a large number of clinically relevant drugs.
How rhodamine 123 works?
Rhodamine 123 /ˈroʊdəmiːn/ is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers.
What does rhodamine 123 stain?
Rhodamine 123 is a popular green fluorescent mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. It is widely used in flow cytometry studies involving mitochondrial membrane potential.
Is rhodamine a fluorophore?
Rhodamine core optical fluorophores are widely utilized in biomedical research, and among the green fluorescent dyes, Rhodamine Green has been shown to possess the highest emission efficiency and the longest sustained fluorescence even after cellular internalization (6).
Is rhodamine cell permeable?
In other words, the cell membrane permeability of rhodamine is excellent. In a review about fluorescent probes for living cells,19 delivery was considered as the most essential character.
How do you make a cell permeable?
Two general types of reagents are commonly used: organic solvents, such as methanol and acetone, and detergents such as saponin, Triton X-100 and Tween-20. The organic solvents dissolve lipids from cell membranes making them permeable to antibodies.
How is rhodamine 123 transported across the intestine?
Transport of rhodamine 123, a P-glycoprotein substrate, across rat intestine and Caco-2 cell monolayers in the presence of cytochrome P-450 3A-related compounds
How is the fluorescent dye rhodamine 123 used?
The potentiometric fluorescent dye rhodamine 123 (Molecular Probes, Eugene, OR) is used in this assay to provide a relative assessment of electrochemical potential across the mitochondrial membrane.
How is the transport of rho-123 inhibited?
Similarly, transepithelial transport of Rho-123 from the basolateral to apical side across Caco-2 cell monolayers was inhibited by these compounds. A linear relationship was observed in their inhibitory potencies on Rho-123 transport between in vitro and in vivo studies using rat ileum and between studies with rat ileum and Caco-2 cells.
How is rhodamine used in the bioenergetics field?
It is used to assess mitochondrial bioenergetics in living cells and to measure the efflux activity of ATP BINDING CASSETTE TRANSPORTER, SUBFAMILY B, MEMBER 1 in both normal and malignant cells. (Leukemia 1997;11 (7):1124-30)