What is Protein A Sepharose?
What is Protein A Sepharose?
Protein A is a genetically engineered protein containing five IgG-binding regions of native Protein A. Protein A Sepharose® beads display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation.
What is Sepharose 4B?
Sepharose® 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Presence of 3,6-anhydro-L-galactose resists biological degradation. Hydrogen bonds stabilize the sepharose gel structure. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.
What Sepharose beads?
Sepharose is a tradename for a crosslinked, beaded-form of agarose, a polysaccharide polymer material extracted from seaweed. Its brand name is a portmanteau derived from Separation-Pharmacia-Agarose. A common application for the material is in chromatographic separations of biomolecules.
What is protein A used for?
Protein A is often immobilized onto a solid support and used as reliable method for purifying total IgG from crude protein mixtures such as serum or ascites fluid, or coupled with one of the above markers to detect the presence of antibodies.
How does protein A bind to antibodies?
Protein A is a 42-kDa protein found in the cell wall of Staphylococcus aureus. It binds with high affinity the Fc region of immunoglobulins from various species (8). There are four binding sites for antibodies but only two of them can be used simultaneously.
What is Sepharose 6B?
Sepharose 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
How is protein A-Sepharose 4B prepared for research?
This product is prepared by covalently coupling highly purified (>98% by SDS-PAGE) Staph. aureus Cowan strain 1 Protein A to Sepharose® 4B. This product is supplied as a 50% suspension consisting of 25ml Protein A-Sepharose® 4B and 25 ml of PBS (10 mM phosphate-buffered saline, pH 7.4, containing 0.19% Kathon CG/ICP). For Research Use Only.
What can protein a sepharose beads be used for?
Protein A Sepharose ® beads display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation. Store at +4°C.
How much protein is in a protein sepharose column?
Ready-to-use pre-packed columns of 1 mL or 5 mL bead volume in PBS with 0.02% sodium azide; >5mg Protein A/mL Sepharose beads. High binding capacity = Binding of IgG ≥16 mg human or rabbit IgG/mL Protein A-Sepharose ®.
What kind of chromatin is in Sepharose 4B?
| Chromatin Biology Column Type Affinity Column Product Line Sepharose™ Shipping Condition Wet Ice Stationary Phase Protein A Format Suspension