What is attB?
attB: Stands for bacterial attachment site which helps in site specific recombination and always attaches with the PCR product. It consist of four homologous core sequence of ~25 bp each, attB1 ACAAGTTTGTACAAAAAAGCAGGCT.
What is LR reaction?
The LR Reaction, again is a recombination reaction between attL and attR sites. The reaction generates an expression clone and is catalyzed by recombinant proteins. Recombination between these sites generates two molecules. One molecule contains the DNA segment of interest, the other molecule is a by-product.
When why AC is used to clone DNA What is the size of the DNA that can be cloned?
1. When YAC is used to clone DNA. What is the size of the DNA that can be cloned? Explanation: YACs are sophisticated cloning vectors that are used for propagating large stretches of DNA which are upto a few megabases.
What is attP and attB?
The attP site (5′-GTGCCCCAACTGGGGTAACCTttGAGTTCTCTCAGTTGGGGG-3′) is one of the two target sites of the phiC31:int integrase. phiC31:int mediates recombination between two non-identical target sites, attP and attB, each of which contains a short integration core flanked by imperfect inverted repeats.
What is a gateway cassette?
A Gateway® cassette containing attR recombination sites flanking a ccdB gene (1) and a chloramphenicol-resistance gene are blunt-end cloned into the multiple cloning site of any vector. ccdB Survival 2 competent cells allow propagation of the Gateway® vectors containing the ccdB gene.
Where can you find a plasmid in a cell?
Plasmid. Plasmid. =. A plasmid is a small, often circular DNA molecule found in bacteria and other cells. Plasmids are separate from the bacterial chromosome and replicate independently of it.
Which is an example of plasmid mediated resistance?
An example plasmid with two areas of antibiotic resistance coding DNA (1,2) and an origin of replication (3). Plasmid-mediated resistance is the transfer of antibiotic resistance genes which are carried on plasmids. The plasmids can be transferred between bacteria within the same species or between different species via conjugation.
Which is the correct function of a degradative plasmid?
D is correct. Degradative plasmids code for enzymes that help digest uncommon compounds such as salicylic acid. Choice A is the function of fertility F-plasmids, B is the function of Col plasmids, and C is the function of virulence plasmids.
Can a plasmid have an observable effect on the host cell?
Some plasmids, however, have no observable effect on the phenotype of the host cell or its benefit to the host cells cannot be determined, and these plasmids are called cryptic plasmids. Naturally occurring plasmids vary greatly in their physical properties.