What does DRAQ5 stain?
What does DRAQ5 stain?
DRAQ5™ is a far-red emitting, anthraquinone compound that stains nuclei in live cells. It is permeant to live cells and thus can be used for cell cycle analysis and/or staining of nucleated cells. It is optimally excited at 568 nm, 633 nm, and 647nm and can be detected using 695LP, 715LP, and 780LP filters.
Does DRAQ5 stain dead cells?
DRAQ5™ stains intact, live, fixed, permeabilized and dead cells. 6. Cells can be analysed directly without further treatment or washing.
Which dye is most suitable for neuronal cell nucleus staining?
The membrane-permeant Hoechst 33342 dye (H1399, H3570, H21492) has been extensively used for staining the nuclei of live cells.
How does calcein work?
Calcein AM is a non-fluorescent cell-permeable derivate of calcein that is widely used in cell viability measurement. Once inside cell, AM groups are hydrolyzed by intracellular esterases. The fluorescent calcein molecule is restored, which is trapped in the cell and emits strong green fluorescence.
What staining is best for cytoplasm?
Haematoxylin and eosin (H&E) staining Haematoxylin and eosin staining is frequently used in histology to examine thin tissue sections. Haematoxylin stains cell nuclei blue, while eosin stains cytoplasm, connective tissue and other extracellular substances pink or red.
How long does calcein AM last?
We use Calcein AM to allow us to take images of neuronal processes for analysis of their length and branching. Over the short term (12-24 hours) Calcein AM does not seem to kill neurons. Some minimal neuronal death is seen by 48 hrs of culture with Calcein AM and this is increased by 72 hrs culture.
What is Annexin V staining?
Annexin V staining is a common method for detecting apoptotic cells. Thermo Fisher Scientific offers high-quality fluorescent annexin V conjugates as standalone reagents and in a variety of kits for use in flow cytometry and for imaging suspension cells.
How is DRAQ5 dye used in flow cytometry?
In flow cytometry, this dye can be used to distinguish nucleated and non-nucleated cells. DRAQ5™ can also be used to report nuclear DNA content for ploidy and cell cycle analysis because it binds DNA stoichiometrically. In fluorescent microscopy, it can be used as a nuclear counterstain. DRAQ5™ can be excited by wavelengths from 488 to 647 nm.
Which is the best stain for degenerating neurons?
Fluoro-Jade C is the most recent of the Fluoro-Jade stains and has been found to stain all degenerating neurons, regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal-to-background ratio, as well as the highest resolution, therefore giving maximal contrast and affinity for degenerating neurons.
Which is the best wavelength for DRAQ5 to be excited?
DRAQ5™ can be excited by wavelengths from 488 to 647 nm. For microscopy/imaging, 633 or 647 nm sources for excitation are recommended.
Where are pyramidal neurons found in the CNS?
They are found in several regions of the CNS and, although the reasons for their abundance remain unclear, functional studies–especially of CA1 hippocampal and layer V neocortical pyramidal neurons–have offered insights into the functions of their unique cellular architecture.