What does a mixed field reaction mean?

What does a mixed field reaction mean?

In a mixed field reaction, two different ABO groups are present in the same sample, causing discordant ABO typing. The front and back type results are not as predicted.

What can causes a mixed field reactions in blood bank?

Often the cause of the mixed field reactions is easily ascertained on history, when the patient is found to have had recent transfusion or stem cell transplantation from a non-group identical donor.

What is mixed field in blood bank?

In transfusion medicine, mixed-field agglutination refers to mixed reactions during cell typing where two distinct cell populations are present: agglutinated cells admixed with many unagglutinated cells. The presence of two or more cell populations is known as chimerism.

What does an antibody screen detect?

The antibody screening test performed in a clinical laboratory and/or blood bank is designed to detect the presence of unexpected antibodies, especially alloantibodies in the serum to antigens of the non-ABO blood group system: Duffy, Kell, Kidd, MNS, P, and certain Rh types that are considered clinically significant.

What happens when blood of different groups is mixed?

A person with type A blood receiving a transfusion of type B or AB blood would have an ABO incompatibility reaction. In an ABO incompatibility reaction, your immune system attacks the new blood cells and destroys them. The two samples of blood are then mixed and watched for a reaction.

What does a positive antibody test mean?

A positive antibody test result shows you may have antibodies from a previous infection or from vaccination for the virus that causes COVID-19. Some antibodies made for the virus that causes COVID-19 provide protection from getting infected.

What causes mixed field reaction with anti M?

Knowing all the possible causes of mixed field reaction would help to resolve and interpret the results accordingly. Anti-M is generally a cold reacting antibody and its reaction strength may vary because of dosage. Antibodies to Lutheran and Sd a antigens may show mixed field appearance but it is unusual for anti M antibodies. [1]

Is the mixed field in plasma a valid result?

Mixed field in plasma/serum testing? Is mixed field a valid result for an antibody screen done by gel method? If so, under what circumstances would that be reported? I found an older thread regarding the degradation of antigens on stored reagent cells yielding such a reaction, but these were relatively fresh screening cells.

What happens if the antibody screen is negative?

If the screen is negative, there is a very high likelihood that no significant antibodies are present (though some rare antibodies against low-incidence RBC antigens could still be present). Important Caveat: Don’t let me catch you saying something silly like, “The antibody screen is negative, so there are no antibodies!”

When to use column agglutination for mixed field reaction?

In both the cases, we observed mixed field reaction during pretransfusion testing using column agglutination technology [Figure 1]. Relevant patient’s history was obtained and further immunohematological work-up was carried out in both the cases to look for the possible cause.