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How does BCP assay work?

How does BCP assay work?

The BCP Albumin Assay Kit is designed to measure albumin directly without any pretreatment of samples, such as serum, plasma, urine, and biological preparations. The intensity of the color, measured at 610 nm, is directly proportional to the albumin concentration in the sample.

What is the function of bromocresol green indicator?

Bromocresol green (BCG) is a dye of the triphenylmethane family (triarylmethane dyes). It belongs to a class of dyes called sulfonephthaleins. It is used as a pH indicator in applications such as growth mediums for microorganisms and titrations. In clinical practise, it is commonly used as a diagnostic technique.

What is the pKa of bromocresol purple?

In its cyclic sulfonate ester form, it has a pKa value of 6.3, and is usually prepared as a 0.04% aqueous solution.

What is albumin BCP?

The Albumin BCP assay is used for the quantitation of albumin in human serum or plasma. SUMMARY AND EXPLANATION OF TEST. Albumin is the major serum protein in normal individuals. Elevated serum albumin levels are usually the result of dehydration.

What color is bromocresol green in basic solutions?

Indicator Range

Indicator Colour
Acid Base
Thymol Blue – 1st change red yellow
Methyl Orange red yellow
Bromocresol Green yellow blue

What happens if bromocresol green turns blue?

In aqueous solution, Bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher pH to give the dianionic form (blue), which is stabilized by resonance(1). It becomes yellow at acidic pH level (pH 3.8) and Blue -green at from pH 5.4.

Why is the basic form of bromocresol purple violet?

Why is the basic form of bromocresol purple violet, whereas the acidic form is yellow? : A) The basic form has less p-orbitals overlapping to form pi molecular orbitals and thus has smaller conjugation than the acidic form. The more p-orbitals that participate in a conjugated.

What is the pH range of bromocresol green?

Prepare bromocresol green solution, 0.1 g in 14.3 mL of M NaOH + 235.7 mL of water. See: pH (Commercial). The pH tests use an indicator that changes colour with changes in the concentration of hydrogen ions, or the acidity of the solution….

Indicator 5.0 Bromocresol green
pH Range 3.8-5.4
Acid yellow
Alkali blue

What causes high levels of albumin in the blood?

Abnormally high levels of albumin most often occur as a result of dehydration, which may be caused by other conditions such as severe diarrhea. Doctors often interpret the significance of albumin levels alongside measurements of other indicators of metabolism and the health of the liver and kidneys.

Is there an overestimation of albumin by Bromocresol purple?

Overestimation of Albumin Measured by Bromocresol Green vs Bromocresol Purple Method: Influence of Acute-Phase Globulins Albumin results from the BCP and BCG methods may result in unacceptable differences and clinical confusion, especially at lower albumin concentrations.

Which is the best method to measure serum albumin?

Background: Usually serum albumin is measured with dye-binding assay as bromocresol green (BCG) and bromocresol purple (BCP) methods. The aim of this paper was to examine the differences in albumin measurements between the Advia2400 BCG method (AlbBCG), Dimension RxL BCP (AlbBCP) and capillary zone electrophoresis (CZE).

Which is better Alb BCP or bromcresol Green?

Albumin measured by a bromcresol purple dye‐binding assay (Alb BCP) agrees more closely with the gold standard of immunonephelometry than does bromcresol green (Alb BCG) measurement. Both tests are in current clinical use. A method for converting between the two would be useful. Methods.

What makes Bromocresol purple as a pH indicator?

Bromocresol purple is a member of the class of 2,1-benzoxathioles that is 2,1-benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3-bromo-4-hydroxy-5-methylphenyl groups. A hydrophilic dye that is used as a pH indicator and to measure serum albumin concentrations.