How do you stain a blood smear with Diff Quick?
How do you stain a blood smear with Diff Quick?
The Diff-Quik stain consists of a fixative agent (methanol, blue), solution I (eosinophilic, orange) and solution II (basophilic, blue). Generally, slides are dipped sequentially into each solution 6 times (or left for 10-15 seconds in each solution), followed by a water rinse and drying.
Which method is used for staining of blood cells?
Routine analysis of blood in medical laboratories is usually performed on blood films stained with Romanowsky stains such as Wright’s stain, Giemsa stain, or Diff-Quik. Wright-Giemsa combination stain is also a popular choice.
Which stain is used for peripheral blood smear?
Commonly used stain in our environment is Leishman stain which is composed of polychrome methylene blue (basic component) and eosin (acidic component). May-Grunwald Giemsa or Wright-Giemsa stain can also be used.
How do you use Diff Quick?
Method
- Allow smears to dry.
- Dip slide or tape-strip five times, for one second each, into Fixative.
- Dip slide or tape-strip five times, for one second each, into Stain 1.
- Dip slide or tape-strip five times, for one second each, into Stain 2.
- Rinse slide or tape-strip in distilled water or Weise’s buffer, pH 7.2.
What kind of stain is diff quick?
Romanowsky stain
Diff-Quik is a commercial Romanowsky stain variant used to rapidly stain and differentiate a variety of pathology specimens. It is most frequently used for blood films and cytopathological smears, including fine needle aspirates.
What are the 3 factors to consider when performing a peripheral blood smear?
The perfect quality smear is influ- enced by three factors: speed, angle and drop size.
Why is a peripheral blood smear done?
A blood smear, also referred to as a peripheral smear for morphology, is an important test for evaluating blood-related problems, such as those in red blood cells, white blood cells, or platelets.
What are staining techniques?
Types of Staining Techniques
| Sr. No. | Staining Technique |
|---|---|
| 1. | Simple (Monochrome) |
| 2. | Negative (Relief) |
| 3 | Gram |
| 4 | Acid fast (Ziehl-Neelsen technique) |
What are the three methods of preparing a blood smear?
Four different types of smear preparation methods (conventional method, blood film method, drop and rest method, and water-wash method) were carried out according to the standard reference as described below. A drop of sample was placed onto a clean slide and then spread to make a smear of 1 cm diameter.
How do you dispose of Diff Quick stain?
Collect liquid in an appropriate container or absorb with an inert material (e.g. vermiculite, dry sand, earth), and place in a suitable container for reclamation or disposal. Do not use combustible materials, such as sawdust. Do not flush to sewer.
Which is the best differential stain for blood smears?
The Quink® differential staining of blood smears appears to work best using undiluted or twofold diluted Quink®. Mizutani (7) was able to stain the nuclei of herpes giant cells using a 5% solution (twenty fold dilution) of Quink® in phosphate buffered saline.
Which is the best ink for differential staining?
Fountain pen ink is a known stain, both of writer’s fingers and samples for the microscope. I therefore tried a quick stain with Parker’s Quink® black ink and was surprised by the increased contrast obtained, for erythrocytes (red blood cells), platelets and the differential staining of five different types of white blood cells that I could find.
How to make a smear of peripheral blood?
Smears are rapidly air-dried, labeled in pencil along the base of the blood smear, and stained in a routine manner. Fig. 14-2 Steps in producing coverslip blood smear.A, Drop of blood is placed in center of coverslip, using microhematocrit tube.
How does Quink stain work in white blood cells?
The stain is simple to use and consistent. Quink® appears to make red blood cells (erythrocytes) more visible by weakly binding to the outer surface. In white blood cells, the stain penetrates into the cells and preferentially stains nuclear material, with weaker staining of the cytoplasm.