How do you get air out of HPLC pump?

How do you get air out of HPLC pump?

Treatment 1: Open a purge valve. Prime the pump with degassed solvent. Treatment 2: Open the compression fitting at the top of the outlet check valve with a slow pump flow until solvent leaks around the fitting. Tap the side of the check valve with a small wrench until small air bubbles come out with the liquid.

How do I purge Agilent HPLC?

Use new or different mobile phase (as required). Purge each channel with 2.5 – 3 mL/min for 5 min. Open the purge valve (1260) or use the purge command (1290). Equilibrate your system with composition of your application for 15 min.

What is back pressure in HPLC?

High Back Pressure is defined as an unexpected increase in the pressure readings during normal HPLC operation that approach or exceed the maximum pressure capability of the system. Higher than expected pressure is the most often reported problem in HPLC.

What to do when HPLC runs dry?

11 If your solvent reservoir runs dry, do not despair. HPLC pumps do not pump air. Simply remove the column from the system, prime the pump and displace any air in the system before connecting the column. Most standard HPLC columns do not suffer from some air passing through them.

How do I clean my HPLC system?


  1. Change the mobile phase to filtered distilled water.
  2. Allow the water to flow through the column at the rate of 1ml / min.
  3. Change the mobile phase from water to HPLC grade 80% Acetonitrile.
  4. Set the instrument to flow rate 1 ml/ minute and wash the column for 30 minutes.

What causes pressure fluctuations in HPLC?

HPLC Pump or System Pressure Fluctuation Causes and Solutions: Air In the Liquid or Mobile phase — Air gets into the system due to a leak or from gas trapped in the mobile phase. Leaks are the most common cause of instability, but insufficiently degassed solution is a close second.

What is purging in HPLC?

“When setting up an HPLC system, the aim of the purge is simply to flush through all the lines so that any remaining solvent in them from a previous analysis or wash is replaced with the new mobile phase. A flow rate of 5mL/min is commonly used for standard HPLC systems.

What are the tips and tricks of HPLC troubleshooting?

Tips and Tricks of HPLC System Troubleshooting Agilent Technologies, Inc. LC Tips And Tricks Seminar Series Page 2 Trouble Shooting Steps You Have Recognized There is a Problem! How Do You Fix It? •1stDid System Suitability or Sample Fail? •2ndReview Method for Compliance –Is The Procedure Being Followed Properly?

What to know about the Agilent 1260 Infinity isocratic pump?

This manual covers: †the Agilent 1260 Infinity Isocratic Pump (G1310B) and †the Agilent 1260 Infinity Quaternary Pump (G1311B) 1 Introduction This chapter gives an introduction to the module, instrument overview and internal connectors 2 Site Requirements and Specifications

What do you need to know about Agilent 1200 series pump?

Agilent 1200 Series Pump Models – Analytical Isocratic Pump Quaternary Pump Std Binary Pump Page 12 Pump Head – Main Components Plunger housing Pump head Outlet ball valve Active inlet valve Purge valve Page 13 Pump and Degasser Maintenance • Clean the degasser lines by flushing with isopropanol.

When to use Agilent 1260 Infinity high performance micro autosampler?

11/2013 Printed in Germany Agilent Technologies Hewlett-Packard-Strasse 8 76337 Waldbronn This product may be used as a com- ponent of an in vitro diagnostic sys- tem if the system is registered with the appropriate authorities and com- plies with the relevant regulations. Otherwise, it is intended only for gen- eral laboratory use. Warranty