Are THP-1 cells suspension cells?
Are THP-1 cells suspension cells?
Normally THP-1 cells grow in suspension, but they can be induced to differentiate into adherent macrophage-like cells, which express IL-8 and other markers, and can thus serve as a model system for differentiation.
How do you revive thp1 cells?
For one vial of cells we rapidly thaw them in a water bath and add 1ml of cells to 25-30 ml of the medium in an up right T-25 flask. The cells are placed in the incubator in the upright position (cap up) for 3-4 hrs. This will allow the cells to settle to the bottom of the flask.
What is raw cell line?
RAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This cell line is a commonly used model of mouse macrophages for the study of cellular responses to microbes and their products. RAW-Blue™ cells express the secreted alkaline phosphatase (SEAP) reporter gene.
How are THP-1 cells used as in vitro cells?
THP-1 cells have been widely used as an in vitro model of human monocytes and macrophages in mechanistic studies of inflammatory diseases [2], [3], [4], [6]. It has long been believed that inflammatory monocytes are recruited and differentiated into macrophages at the site of infection [81].
How many CD4 cells are in a THP1 cell line?
An amount of liposomes containing 10 μg⧸ml CD4 is incubated with uninfected H9 or THP-1 cells, or the chronically infected H9⧸HTLV-IIIB or THP-1⧸HIV-1 IIIB cells, for 1 h at 37° [10 6 viable cells in a final volume of 100 μl of RPMI⧸0.2% bovine serum albumin (BSA)] ( Fig. 4 ).
How are THP-1 cells differentiated into macrophages?
THP-1 morphology and surface receptor expression. THP-1 cells were differentiated into macrophage-like cells (THP-1 macrophages) by incubation in the presence of PMA, which leads to a macrophage-like phenotype characterized by changes in morphology and increased cell surface expression of CD11 and CD14 [10,11,14,17,21].
Where do you get THP1 cell lines from?
Specific binding of the CD4-liposomes to HIV-infected cells is determined by using chronically infected cell lines. THP-1 cells are obtained from the American Type Culture Collection.