Where does site-specific recombination occur?
Site-specific recombination is an exchange that occurs between pairs of defined sequences (target sites) residing on the same DNA molecule or on two different DNA molecules. The result of the exchange may be integration, excision, or inversion of DNA sequences.
Which of following is site-specific recombination?
2. Which of the following is a must for site-specific recombination reaction? Explanation: Recombinase is a tetrameric protein that binds to the two chromosomes undergoing site-specific recombination reaction. It is a must for the reaction.
What are the two families of conservative site-specific recombination?
Only two classes of conservative site-specific recombinases have been discovered so far: the invertase/resolvase family (also called the serine family) and the lambda integrase family (also called the tyrosine family).
Are transposons site-specific?
Transposons, also called transposable elements, are mobile genetic elements that generally have only modest target site selectivity and can thus insert themselves into many different DNA sites. Those in the first two of these classes use virtually identical DNA breakage and DNA joining reactions to translocate.
What are the types of recombination?
There are three types of recombination; Radiative, Defect, and Auger. Auger and Defect recombination dominate in silicon-based solar cells. Among other factors, recombination is associated with the lifetime of the material, and thus of the solar cell.
What is Transpositional site-specific recombination?
Transposition is the process by which genetic elements move between different locations of the genome, whereas site-specific recombination is a reaction in which DNA strands are broken and exchanged at precise positions of two target DNA loci to achieve determined biological function.
What are the 2 types of recombination?
At least four types of naturally occurring recombination have been identified in living organisms: (1) General or homologous recombination, (2) Illegitimate or nonhomologous recombination, (3) Site-specific recombination, and (4) replicative recombination.
What is an example of recombination?
Recombination in meiosis. Recombination occurs when two molecules of DNA exchange pieces of their genetic material with each other. One of the most notable examples of recombination takes place during meiosis (specifically, during prophase I), when homologous chromosomes line up in pairs and swap segments of DNA.
What is the P
The P element encodes for the protein P transposase. Unlike laboratory strain females, wild type females are thought also to express an inhibitor to P transposase function, from the very same element. This inhibitor reduces the disruption to the genome caused by the P elements, allowing fertile progeny.
What are different types of recombination?
Which is the best description of site specific recombination?
Site-specific recombination, also known as conservative site-specific recombination, is a type of genetic recombination in which DNA strand exchange takes place between segments possessing at least a certain degree of sequence homology.
How does CRE play a role in site specific recombination?
Cre recombinase is a tyrosine recombinase enzyme using a topoisomerase I-like mechanism to bring about site-specific recombination events. In the life cycle of the P1 bacteriophage, enzymes play an important role, such as cyclization of the linear genome and resolution of dimeric chromosomes (Van Duyne, 2001 ).
Where does site specific recombination lead to DNA inversion?
Site-specific recombination leading to DNA inversion is widespread in both Gram-positive and Gram-negative bacteria. Inversion has been shown to occur not only on chromosomes, but has also been found on plasmids and bacteriophages with inverted DNA sizes ranging from as little as 314 bp to 35 kb.
How are the arms of the recombination cycle different?
As both arms are structured slightly differently, the subunits become conformationally tuned and thereby prepared for their respective role in the recombination cycle. Contrary to members of the Tyr-class the recombination pathway converts two different substrate sites (attP and attB) to site-hybrids (attL and attR).