What is the capacity factor in HPLC?
What is the capacity factor in HPLC?
The capacity factors tells the extent to which your component will bind to the column with respect to your unretained components or you can say first baseline disturbance. if the drug interacts in the column you have some value as per the formula provided by Subrata Bhadra sir so you can say the drug is binding.
What does HPLC measure?
High-performance liquid chromatography or high-pressure liquid chromatography (HPLC) is a chromatographic method that is used to separate a mixture of compounds in analytical chemistry and biochemistry so as to identify, quantify or purify the individual components of the mixture.
What factors affect HPLC?
the pressure used (because that affects the flow rate of the solvent) the nature of the stationary phase (not only what material it is made of, but also particle size) the exact composition of the solvent. the temperature of the column.
How HPLC is used as a separation technique?
HPLC relies on pumps to pass a pressurized liquid and a sample mixture through a column filled with adsorbent, leading to the separation of the sample components. The active component of the column, the adsorbent, is typically a granular material made of solid particles (e.g., silica, polymers, etc.), 2–50 μm in size.
What is capacity factor formula?
The capacity factor is the average power generated, divided by the rated peak power. If it produces power at an average of two megawatts, then its capacity factor is 40% (2÷5 = 0.40, i.e. 40%). To calculate the average power generated, just divide the total electricity generated, by the number of hours.
How is capacity factor calculated?
Capacity factor is the measure of how often a power plant runs for a specific period of time. It’s expressed as a percentage and calculated by dividing the actual unit electricity output by the maximum possible output. This ratio is important because it indicates how fully a unit’s capacity is used.
What are the advantages and disadvantages of HPLC?
Disadvantages & Advantages of an HPLC
- HPLC and Similar Techniques. Like other forms of chromatography, HPLC allows the separation of chemical constituents through the use of a mobile phase and a stationary phase.
- Speed, Efficiency and Accuracy.
- Cost and Complexity.
- Sensitivity and Resolution.
What is the basic principle of HPLC?
The separation principle of HPLC is based on the distribution of the analyte (sample) between a mobile phase (eluent) and a stationary phase (packing material of the column). Depending on the chemical structure of the analyte, the molecules are retarded while passing the stationary phase.
Why pH is important in HPLC?
When samples contain ionisable compounds, mobile phase pH can be one of the most important variables in the control of retention in a reversed‑phase HPLC (RP-HPLC) separation. Since most compounds analysed by RP-HPLC contain one or more acidic or basic functional groups, most mobile phases require pH control.
What are the limitations of HPLC?
What Are the Disadvantages of HPLC?
- Coelution. Because of the speed of HPLC and it reliance on different polarities of compounds, two compounds with similar structure and polarities can exit the chromatography apparatus at the same time or nearly the same time.
- Adsorbed Compounds.
- Cost.
- Complexity.
What is a good retention factor?
For more complex mixtures, the useful retention factor range is typically 2 < k < 10. Theoretically, you can keep increasing the retention factor as you wish. But higher k values come at a cost, just as I need more supplies and more time to work with a higher canvas size when painting.
What are the disadvantages of HPLC?
The disadvantage of HPLC: HPLC can be an expensive method, it required a large number of expensive organics, needs a power supply, and regular maintenance is required. It can be complicated to troubleshoot problems or develop new methods.
What is difference between HPLC and TLC?
The key difference between TLC and HPTLC is that the absorbent material in TLC plate has large particles whereas HPTLC plates have very small particles of adsorbent material. TLC is thin layer chromatography.
What is basic principle of HPLC?
HPLC Theory. HPLC works following the basic principle of thin layer chromatography or column chromatography, where it has a stationary phase ( solid like silica gel) and a mobile phase (liquid or gas). The mobile phase flows through the stationary phase and carries the components of the mixture with it.
What factors affect HPLC column lifetime?
What factors affect column lifetime? Commonly reported HPLC problems include slow flow rate and increased column back pressure. Either of these observations could indicate that a column’s performance is being compromised.