What is micellar electrokinetic capillary chromatography?
Micellar electrokinetic chromatography is a modified version of capillary electrophoresis which is used to separate a wide range of molecules, from inorganic ions to large biopolymers.
What is the principle of micellar electrokinetic capillary chromatography how does it differ from CZE?
MEKC differs from CZE because it uses an ionic micellar solution instead of the simple buffer salt solution. MEKC can be used for the separation of both ionic and neutral substances while CZE typically separates only ionic substances.
What is the main application of micellar electrokinetic chromatography MEKC technique?
Recent applications of MEKC include the analysis of uncharged pesticides, essential and branched-chain amino acids in nutraceutical products, hydrocarbon and alcohol contents of the marjoram herb. MEKC has also been targeted for its potential to be used in combinatorial chemical analysis.
Is capillary electrophoresis chromatography?
The heart of capillary electrophoresis (CE) is electroosmotic flow (EOF). This is the mobile phase “pump” in CE. Unlike gas chromatography (GC), there is no pressurized gas acting as the mobile phase in CE. Unlike high performance liquid chromatography (HPLC) these is no (high pressure) pumped mobile phase.
Why can micellar electrokinetic chromatography be used to separate neutral molecules?
Transcribed image text: Why can micellar electrokinetic chromatography separate neutral molecules? The micelles add an element of equilibration time to the van Deemter equation, which helps increase plate height to create a broader range where neutral molecules can be resolved m separations.
What is a capillary gel?
Capillary Gel Electrophoresis (CGE) is an analytical separation method where charged molecules are separated in capillaries filled with porous gel matrix. CGE is used to separate large biological molecules like protein, DNA and RNA.
Why does electroosmotic flow occur?
Electroosmotic flow occurs when an applied driving voltage interacts with the net charge in the electrical double layer near the liquid/solid interface resulting in a local net body force that induces the bulk liquid motion.
What is isoelectric focusing and its application?
IEF is used mainly to separate proteins for analysis or purification. It measures the isoelectric points (pI) of proteins and uses the unique pI values of proteins to purify them. The pI of any particular protein is defined as the specific pH at which it carries no net electrical charge.
Which method is best for the separation of CE?
Capillary electrophoresis is used most predominately because it gives faster results and provides high resolution separation. It is a useful technique because there is a large range of detection methods available.
What is the difference between capillary electrophoresis and gel electrophoresis?
The key difference between capillary electrophoresis and gel electrophoresis is that gel electrophoresis is performed in a vertical or horizontal plane using a polymer gel of standard pore size whereas capillary electrophoresis is performed in a capillary tube with a polymer liquid or a gel.
What is micellar electrokinetic chromatography used for?
What is Micellar Electrokinetic Chromatography? Micellar electrokinetic chromatography is a modified version of capillary electrophoresis which is used to separate a wide range of molecules, from inorganic ions to large biopolymers.
When did Armstrong and Henry invent micellar chromatography?
In 1980, Armstrong and Henry pioneered the use of micellar solutions as mobile phases for reversed-phased liquid chromatography (RPLC). In the literature, MEKC is also often referred to as MECC (micellar electrokinetic capillary chromatography) since the separations are most often performed in a capillary tube.
Which is a mode of electrokinetic chromatography ( EKC )?
Electrokinetic chromatography (EKC) is a family of electrophoresis tech- niques named after electrokinetic phenomena, which include electroosmo- sis, electrophoresis, and chromatography. Micellar electrokinetic chromatography (MEKC) is a mode of EKC in which surfactants (mi- celles) are added to the buffer solution.
How is critical micelle concentration used in chromatography?
The critical micelle concentration was first observed by Mc Bain where at a certain concentration, the surfactants tend to aggregate. These surfactant aggregates can adopt a variety of shapes and sizes based on the concentration of surfactants, cp-solvents, pH, temperature and pressure.