What is BioID?
What is BioID?
BioID is a unique method to screen for physiologically relevant protein interactions that occur in living cells. This technique harnesses a promiscuous biotin ligase to biotinylate proteins based on proximity. Proteins identified by BioID are candidate interactors for the protein of interest.
What is proximity biotinylation?
Proximity biotinylation based on Escherichia coli BirA enzymes such as BioID (BirA*) and TurboID is a key technology for identifying proteins that interact with a target protein in a cell or organism. These results indicate that AirID is a novel enzyme for analyzing protein–protein interactions.
What is proximity-dependent biotin identification?
Proximity-dependent biotin identification (BioID), reported by Roux and colleagues, is a powerful discovery tool for lamin-associated proteins. In this method, living cells express a bait protein (e.g., lamin) fused to an R118G-mutated version of BirA, an Escherichia coli biotinylase.
What is BioID connect?
Biometric authentication software Trusted by customers worldwide, the BioID Web Service provides industry-leading liveness detection and facial recognition APIs. BioID’s software-based biometrics enable seamless user experience, requiring a few selfies only. BioID liveness detection is compliant with ISO/IEC 30107-3.
How are bioID-based biotinylation techniques used in living cells?
Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of cellular compartments in living cells 5, 6, 7. Here we present a BioID-based map of a human cell on the basis of 192 subcellular markers, and define the intracellular locations of 4,145 unique proteins in HEK293 cells.
How is BioID used to screen for protein interactions?
The ligase is fused to a protein of interest and expressed in cells, where it biotinylates proximal endogenous proteins. Because it is a rare protein modification in nature, biotinylation of these endogenous proteins by BioID fusion proteins enables their selective isolation and identification with standard biotin-affinity capture.
How does bioID work with biotin ligase mutations?
In BioID, a wild-type biotin ligase BirA has stringent specificity for its substrate, but its adopted mutation, BirA*, affects biotin ligase activity (disordered loop) rendering it a promiscuous biotinylation enzyme and can covalently modify primary amines in the immediate vicinity of BirA*.
What kind of biotin is used in bioID?
Creative Biolabs provides a novel technique, proximity-dependent biotin identification approach, recently described as BioID, for screening and identification of protein-protein interactions in vivo.