What enzymes are involved in DNA replication and transcription?

What enzymes are involved in DNA replication and transcription?

Enzymes involved in DNA replication are:

  • Helicase (unwinds the DNA double helix)
  • Gyrase (relieves the buildup of torque during unwinding)
  • Primase (lays down RNA primers)
  • DNA polymerase III (main DNA synthesis enzyme)
  • DNA polymerase I (replaces RNA primers with DNA)
  • Ligase (fills in the gaps)

What enzymes are used in RNA transcription?

Transcription is carried out by an enzyme called RNA polymerase and a number of accessory proteins called transcription factors. Transcription factors can bind to specific DNA sequences called enhancer and promoter sequences in order to recruit RNA polymerase to an appropriate transcription site.

How do enzymes work in DNA replication?

The enzyme, DNA Helicase breaks the hydrogen bonds between the bases from the 5′ to 3′ direction, uncoiling the DNA and separating the strands. Thus, allowing other enzymes involved in the process to access each strand of DNA.

What enzymes are important for DNA replication?

DNA replication requires other enzymes in addition to DNA polymerase, including DNA primase, DNA helicase, DNA ligase, and topoisomerase.

How are enzymes and proteins involved in DNA replication?

A number of enzymes and proteins are associated with the replication fork to help in the initiation and continuation of DNA synthesis, Most prominently, DNA polymerase synthesizes the new strands by adding nucleotides that complement each (template) strand. DNA replication occurs during the S-phase of interphase.

Which is the main enzyme in the process of transcription?

Transcription is the process in which a gene’s DNA sequence is copied (transcribed) to make an RNA molecule. RNA polymerase is the main transcription enzyme. Transcription begins when RNA polymerase binds to a promoter sequence near the beginning of a gene (directly or through helper proteins).

Which is the enzyme that synthesizes RNA primers?

In replication, before DNA polymerase iii can begin synthesizing DNA primers must be present on the template generally short segments of RNA synthesized by an enzyme called “Primases”. DNA primase has molecular weight 60,000 Dalton and contains only single subunit, which functions synthesize RNA primers.

How does RNA polymerase II transcribe genes in the eukaryotic cell?

RNA polymerase II (RNAPII) transcribes the major share of eukaryotic genes. During elongation, the transcription machinery needs to move histones out of the way every time it encounters a nucleosome.