What does an Orbitrap do?
What does an Orbitrap do?
The Orbitrap is an ion trap mass analyzer that consists of two outer electrodes and a central electrode, which enable it to act as both an analyzer and detector. By measuring the oscillation frequencies induced by ions on the outer electrodes, the mass spectra of the ions are acquired using image current detection.
What property of the ions does an Orbitrap use for detection?
In such instruments, ions are trapped in a strong magnetic field combined with a weak electric field. Image current from coherently excited trapped ions is detected, digitized, and converted using Fourier transform into the frequency domain and then mass spectra.
What is the advantage of a high resolution instrument?
High resolution data in full scan mode can provide sufficient specificity and selectivity, so that it can be used for quantification without product ion confirmation. This allows faster method development time with minimal need for compound optimization and is especially helpful for analyses involving multiple targets.
Why is high resolution of interest in mass spectrometry?
High-resolution mass spectrometry (HRMS) provides the highest possible precision of m/z measurement using different analyzers such as time of flight, Orbitrap [112], or Fourier transform ion cyclotron resonance. Standard resolution is usually at least 20 times higher than in low-resolution MS systems [95].
What is high resolution mass?
High-resolution mass spectrometry (HRMS) uses mass spectrometers capable of high resolution, as well as high mass accuracy measurements. These instruments can be used to distinguish between compounds with the same nominal mass, determine elemental compositions, and identify unknowns.
How does a linear ion trap work?
How it works. The LIT uses a set of quadrupole rods to confine ions radially and a static electrical potential on the end electrodes to confine the ions axially. The LIT can be used as a mass filter or as a trap by creating a potential well for the ions along the axis of the trap.
What are the high resolution measurements of Orbitrap?
Orbitrap technology drives high-resolution, accurate mass (HRAM) data, generating incredibly high resolution measurements of up to 500,000 FWHM (with the option of achieving up to 1,000,000 FWHM) at m/z 200.
What are the benefits of orbitrap mass spectrometry?
Key benefits of Orbitrap mass spectrometry. Unparalleled sensitivity, resolution, and accuracy. Orbitrap technology drives high-resolution, accurate mass (HRAM) data, generating incredibly high resolution measurements of up to 500,000 FWHM (with the option of achieving up to 1,000,000 FWHM) at m/z 200.
What can Orbitrap technology be used for in forensics?
With its ease of use, robustness and performance, Orbitrap technology is now utilized more widely for applications in forensic toxicology, food safety, drug monitoring research, pharmacokinetics, environmental analysis, as well as various qualitative analyses in pharma and academia.
What is the local minimum of the orbitrap mass analyzer?
A local minimum at z = 0 and r = R is indicated by an arrow. (Reproduced with permission of Thermo Fisher Scientific.) axial oscillations along the central electrode with a frequency ω . Diagram of the Orbitrap mass analyzer showing a stable spiral trajectory of an ion between the central electrode and the split outer electrodes.